Cardioprotective Effects of Salvianolic Acid A on

Myocardial Ischemia-Reperfusion Injury In Vivo and In Vitro

 

Huaying Fan, Liu Yang, Fenghua Fu, Hui Xu, QinggangMeng, Haibo Zhu, Lirong Teng, Mingyan Yang, Leiming Zhang, Ziliang Zhang, and Ke Liu

 

Abstract

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Salvianolic acid A (SAA), one of the major active components of Danshen that is a traditional Chinese medicine, has been reported to possess protective effect in cardiac diseases and antioxidative activity. This study aims to investigate the cardioprotection of SAA in vivo and in vitro using the model of myocardial ischemia-reperfusion in rat and hydrogen peroxide (H₂O₂)-induced H9c2 rat cardiomyoblasts apoptosis. It was found that SAA significantly limited infarct size of ischemic myocardium when given immediately prior to reperfusion. SAA also significantly suppressed cellular injury and apoptotic cell death. Additionally, the results of western blot and phospho-specific antibody microarray analysis showed that SAA could up-regulate Bcl-2 expression and increase the phosphorylation of proteins such as Akt, p42/p44 extracellular signal-related kinases (Erk1/2), and their related effectors. The phosphorylation of those points was related to suppress apoptosis. In summary, SAA possesses marked protective effect on myocardial ischemia-reperfusion injury, which is related to its ability to reduce myocardial cell apoptosis and damage induced by oxidative stress. The protection is achieved via up-regulation of Bcl-2 expression and affecting protein phosphorylation. These findings indicate that SAA may be of value in cardioprotection during myocardial ischemia-reperfusion injury, which provide pharmacological evidence for clinical application.

 

Copyright © 2011 Fan et al. This is an open access article distributed under the

Creative Commons Attribution License

1.              Introduction

2.              Methods

1.           Animals

2.           Regents and Materials

3.           In Vivo Myocardial Ischemia-Reperfusion Protocol and Evaluation of Infarct Size

4.           Cell Culture and Treatment

5.           Cell Viability Assay and Measurement of SOD Activity

6.           Detection of Apoptosis with Annexin-PI

7.           Western Blot Analysis

8.           Phosphospecific Protein Microarray Analysis

9.           Statistical Analysis

3.              Results

1.           Effect of SAA on Myocardial Infarct Size

2.           Effect of SAA on H₂O₂-Induced Deficiency of Cell Viability

3.           Effect of SAA on SOD Activity inH2O2-Treated H9c2 Cells

4.           SAA Protects H9c2 Cells from H2O2-Induced Apoptosis

5.           Effect of SAA on Expression of Bcl-2 and Bax

6.           Effect of SAA on Phosphorylation Proteins

4.              Discussion

5.              References

 

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